Contact an Account ManagerSolid-phase extraction of antioxidant compounds from commercial cranberry extract and its antiradical activity
Summary
Type: SPE
Application #: 305210
Detection:
Conditions: CHROMABOND® PS-OH-:
1) Isolation of vitamin C
Column conditioning: 3 ml methanol, then 3 ml water
(Do not let run the column dry!)
Sample aspiration: The sample was adjusted to pH 11 with diluted NaOH solution and loaded on the column (fraction 1).
Washing: With 3 ml of 10 % NH4OH (fraction 2), then with 3 ml methanol/water (20:80, v/v) (fraction 3)
Elution: Vitamin C was eluted with 2 x 2 ml methanol/acetone (1:1, v/v) + 5 % acetic acid (fraction 4)
CHROMABOND® C18:
1) Isolation of neutral flavonoids and phenolics
Column conditioning: 8 ml methanol, then 4 ml water (pH 7)
(Do not let run the column dry!)
Sample aspiration: The fraction 1 was adjusted to pH 7 and passed through the column. The sample was loaded onto the preconditioned column (fraction 5).
Washing: With 10 ml water (pH 7) (fraction 6)
Elution: 12 ml methanol (fraction 7)
2) Isolation of acidic phenolics
Column conditioning: 8 ml methanol, then 4 ml of 0.01 M HCl
(Do not let run the column dry!)
Sample aspiration: The fraction 1 was adjusted to pH 2 with 2 M HCl and passed through the column (fraction 8).
Washing: With 5 ml of 0.1 M HCl (fraction 9)
Elution: 12 ml methanol (fraction 10)
Evaporation: Each of fractions 2, 3, 4, 6, 7, 8, 9, 10 and the cranberry extract were evaporated to dryness.
Reconstitution: Fractions were dissolved in 80 % acetone in order to obtain the final concentration of 100 mg/ml.
Authors: Tumbas, V.T. et al.
Source: BIBLID: 1450-7188 (2007) 38, 157-164